Agarose Gel Electrophoresis of DNA Lab Report Example | Topics and Well Written Essays - 1250 words

Agarose Gel Electrophoresis of DNA - Lab Report Example DNA is the double stranded helical structure which carries the genetic information. The study of the DNA molecule will give us the details about life. DNA molecules can be extracted from the cell using the extraction techniques and they are then quantified using the agarose gel electrophoresis. The DNA fragment is separated in the Agarose based on the pore size (that is the concentration of the agarose), the voltage applied, the molecular size of the DNA molecule and the conformation of the DNA. The smaller molecules a move downwards in the gel faster than the larger molecules and the separation occurs based on the size of the DNA. If high voltage is applied, then the migration will be faster but the separation of the DNA fragments will not be clear. (Westermeier 2006). So, 110 volts for 30- 45 minutes is usually applied for the separation of the DNA fragments in the gel. The agarose gel electrophoresis of the DNA molecule showed distinct bands in the lanes 4,5 and 6 indicating that only a single DNA is present in the sample. Introduction: Doeoxy ribo nucleic acid (DNA) is a polymer consisting of base, sugar and a phosphate bond. The sugar is always deoxy- ribose in case of DNA and the base will be a purine or pyrimidine molecule: Adenine, Guanine, Cytosine or Thymine. Phosphate molecule connects the two nucleosides. DNA is a covalently linked structure which is helical in shape. DNA is a double helical structure held together by the hydrogen bonds. DNA carries the genetic information. ... Since DNA are acidic in nature, they migrate towards the positive pole when exposed to an electric field. The pore sixe of agarose sieve is determined by the concentration of agarose. Agarose gels are made with a concentration varying from 0.7- 1.5%. DNA in a neutral solution is negatively charged. (Williamson and Campbell 1997). So if an electric field is applied to the DNA, it will move towards the anode pole from the cathode pole. Based on the fragment size, the rate of migration will be inversely proportional to the fragment size. The smaller fragments will move faster than the larger ones and the distance moved by the fragments are measured by using the molecular marker. Molecular marker is the standard DNA fragment sizes which act as the standards to measure the molecular weight of the DNA fragments. Thus by providing constant voltage into the agarose gel, we are able to separate the DNA fragments based on their molecular weight. (Westermeier 2006). Agarose Gel Electrophoresis of DNA is a very simple and reproducible technique. The mobility of the DNA molecule in neutral solution was independent of the size of the fragment but varied with the ionic strength .The DNA fragments of up to 40 kilo base pair can be separated using agarose gel electrophoresis. (Williamson and Campbell 1997). The DNA fragments get separated based on molecular size, the current applied, concentration of Agarose and the conformation of the DNA. Agarose is a copolymer containing 1,3-linked ?-D-galactose and 1,4-linked 3,6- anhydro-?-L-galactose linked together by the junction zones and joined by the hydrogen bonds. (Stellwagen 2009). A standard ladder is used to identify the size of the fragments. In

Using Scientific Method Research Paper Example | Topics and Well Written Essays - 250 words

Using Scientific Method - Research Paper Example the addition of nitrogen into non-arable soil to make it arable, there are other ways carried out to make it arable, they include; digging and installation of wells and canal, and addition of tree for shading purposes. As the experiment progress, we will record differences when nitrogen fertilizers added to maize into two different soil samples.( Brady & Weil ) The experiment was called mineral nitrogen fertilizer. Various kilograms of nitrogen fertilizers per 100 acres used to get result, fertilizer statistics composed in an increase of 20kg at each acre. In addition to that, a nitrogen fertilizer per 100 acres was added to the yield per every year. As the experiments proceed maize at non-arable soil, grow at higher late than that in arable soil.( Brady & Weil ) According to my hypothesis, I was correct that maize in the soil, which is non-arable, it would grow at a higher rate than on arable soil.( Berlin De Gruyter. ) As the experiment progress, we have noticed that maize in the non-arable soil grow at higher rate than in arable soil, every time nitrogen fertilizer added on it. On the other hand, as the fertilization process came close to 180kg per 100 acres, both were at the equal produce of 20.1mg of dry mass. Perhaps this happened because arable soil has been in use for many years.( Berlin De Gruyter.

Criminological research Essay Example for Free

Criminological research Essay Longitudinal Approach to Chicago Crime Research The Project on Human Development in Chicago Research conducted a research regarding the historical origins of crime using the statistical longitudinal approach — eight-year-follow-up from 1993 to 2001 on 11,000 individuals with equal male to female ratio in nine different age groups selected at random from 70 different communities— and the developmental crime analysis using multi-field approaches from various related studies in criminology, sociology, psychology and biology. The Project is based at Harvard School of Public Health and is jointly sponsored by National Institute of Justice and MacArthur Foundation. The project attempted to explain the psychological and criminological determinants of the city in response to the mounting crime rates from the years 1960’s to current. In lieu with this, the project also aimed to find ‘solutions’ through the aforementioned multi-disciplinary analysis combined with therapeutic intervention analysis. Understanding criminal behavior entails identification of the source (from birth to adulthood), the developmental origin and environmental influences. Main areas for the study include individual differences, influences from family, school, peer and community, criminal careers, predictions of dangerousness. Data collection is by multistream STORI approach—self-reports tests and examinations, observational procedures (surveys and interviews), existing records, and informant reports. Community, as a determinant, is analyzed through systemic social observation. Changes in family structure is measured at several factors including the individual and/or mixed effect of household environment, care providers, key figures , presence of extended family, quality of upbringing, and the relationships within. The impact of racism on construction of identity of the samples was also considered. Gender-specific roles was also investigated–— why males tend to be more violent and the females more into sexual and property offenses  ¬Ã¢â‚¬â€ their differential response to developmental determinants, individuality, and social behavior with focus between adolescence-adulthood period. Antisocial behavior as exhibited by criminal behavior is evaluated at traumatic stress (and PTSD), abuse and child development; the coping mechanism and resilience and cycle of violence hypotheses is evaluated in line with this. Aside from exploring the history and patterns of anti-socialism, the project maimed at creating intervention programs at the following age group to prevent recidivism: 0-6 (improved social skills and cognitive stimulation); and young adolescents (modeling, peer leader and educ films); young adults (probation and diversion programs). Testing persistence-desistence hypothesis by using various theories [ (bio-psych development, social learning and control, social organization, network, rational choice and deterrence theory)] with focus on peer relations will also be included in the Project. The Project also aimed at creating a pragmatic, large-scale approach for crime prevention by testing their hypothesis on differential social organization, individual differences, peer groups and social networks. The Project’s scheme is rather ambitious considering that it requires an 8 year statistical analysis, only 200 field experts and one co-sponsor (aside from the federal). While it is true that ‘meta-analysis’ can be applicable for such types of study, there are problems in statistical method sampling especially if the test samples ‘migrated’ or ‘died’ before the end of the project. Would the Project resort to attrition analysis? The investigation may also be weak because it relies on questionnaires and interviews, and most criminals, in reality, rarely talk about their life. Technology should also be assessed for the study. Additionally, the methods used, although very extensive, may be very tiring to the staff. Although the objectives of the study are great, the methods require extensive staffing and careful management and (detached) association with the samples in the study. Reference Earls, F. J. and Reiss, A. J. (1994). Breaking the Cycle. NIJ Research Report. 91 pp.

Midget Village :: essays research papers

  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Midget Village   Ã‚  Ã‚  Ã‚  Ã‚  One Saturday night, I left the house to go to a friend’s house, and the last words that came out of my parent’s mouth was to be careful. That statement went in one ear and out the other. People never think that anything would ever happen to them because they take their safety for granted. I told them that I am always careful and I’ll be home soon, so I left.   Ã‚  Ã‚  Ã‚  Ã‚  I started up my car and fished around in my back seat for my CD case so I wouldn’t be bored for the twenty-minute ride to my destination. I was driving my Ford Probe that I had just bought two weeks ago. I was feeling like a million dollars in my new car, even though it was a used, it was new to me. I was driving the speed limit and sometimes I was driving slower than it was because I wanted people to see me in it. Since I was doing that, the ride was taking more time than I expected and I knew my friends were waiting for me. Singing to my favorite CD in the car got me a bit thirsty, so I stopped at the seven-eleven to buy a slurpee.   Ã‚  Ã‚  Ã‚  Ã‚  I parked the car and went in. I greeted the people working at the cash register and went into the back of the store and proceeded to get my slurpee. I happened to glance over my shoulder and I saw that the people working in the store were following me around the store as if I was going to steal something. This got me frustrated and mad because that is racial profiling and I don’t like to be judged by the color of my skin. I confronted the employee and asked him flat out, why he was following me around. He told me that people like me come in there all the time and steal. I asked him what he meant by â€Å"people like me?† He didn’t respond, and I got even more frustrated. I put the slurpee in his hands and walked out of the store. I will not buy things from a store that has those kinds of views about minority races. I could have acted like I was one of those people who he was talking about by cussing at him and making a scene, but I just left a nd held everything in.

Electric Cars Are Going to Be the Future Essay

Nearly every household in America owns a gas powered vehicle, which pollutes our environment daily. Our society needs to consider furthering its knowledge and use of electric cars. Pollution of our environment must come to a halt in every situation possible in order to save the future of our atmosphere. I believe that the switch from gas-powered cars to electric cars is a very necessary and reasonable approach to save the environment. Several different car manufacturers have already began making efforts to adapt their vehicles to become more environmentally safe products. Although our country runs off oil and its proceeds, we can still attempt to make conversions that will lead to an overall positive impact on the United States. Making the switch to electric cars that run solely on renewable energy is a very wise economical and green decision. Our environment is long overdue for help in making it a better place for living in. The way we plan to make the environment better for living is by eliminating pollution altogether. The world annually emits approximately 8. 3 billion tons of carbon and about four billion tons will remain in the atmosphere for thousands of years. The switch to electric cars is an easy accomplishment because the technology is already at the tip of our hands. The United States will benefit a great deal after the switch from gasoline cars to electric cars is achieved. After learning the facts, the reasons are oblivious as to why electric cars are more efficient than hydrogen cars. The technology is already prepared for mass production. The negative environmental effects of production and driving the car are minimal. Manufacturers have already succeeded at innovating designs and specific features into the vehicles to assure the public’s satisfaction. With all of this in mind, it is vital to realize that the main goal of individual transportation is to enable people to get where they need to be when they need to be there. Ideally, there should be extremely limited environmental consequences when transporting in a perfect world, there would be none. Therefore, engineers and car manufacturers are not fixing the problem as a whole with their efforts to revolutionize automobiles, but every step forward counts. Instead of being content with the advancements, car manufacturers and engineers should continue to explore the world of environmentally safe opportunities to bring forward into the public eye. One of the most widely known alternatives to gasoline in America is hydrogen or biodiesel. Some might argue that hydrogen-powered or biodiesel-powered cars are more efficient than electric-powered cars, but they are not even close. Hydrogen cars are more expensive to fuel. The hydrogen car costs about three dollars per gallon, which totals around fifteen cents per mile, while the electric car pays in kilowatt-hours, which totals about two cents per mile. The average hydrogen car costs about one million dollars and the technology is just not there yet. It would cost excessively much to install hydrogen fueling stations and biodiesel pumps when it would be much easier and more reasonable to install a greater amount of electrical outlets across the world. Although, electric cars are more expensive to buy off the dealership lot they outperform the regular gasoline cars physically and economically. The electric car overall is cheaper to maintain, but unfortunately, after about ten years the lithium ion batteries start to drain out, but instead of buying a brand new car every so many years all you would have to do is replace the batteries. In the end, this will be a very good deal. The electric alternative technology is already here compared to other alternatives like ethanol, biodiesel, and hydrogen, yes, these are all great ideas, but the technology is just not readily available to us. Therefore, we should not even bother with these alternatives at all. Lastly, the gasoline cars that we use today waste power by constantly burning gas when the car is not in motion, whereas electric cars do not waste power when the car is not in motion.

The Development Of Lifespan Development Essay - 2341 Words

Lifespan Development Report Lifespan development is a particular field of study which encompasses stability and change of behaviour, and examines patterns of growth that occur throughout the entirety of the lifespan of an individual (Feldman 2014, p.5). The study of human development is incredibly important, as it aids us in the understanding of ourselves, our children, our peers and genuinely any member of any society. Development is widespread and culturally diverse, not to mention ever-changing. Therefore, the human race is always providing new areas for research, as well as challenging the conclusions of previous studies. This report aims to analyse a single adult’s experience of development over the period of their life, using qualitative data. This analysis will focus on several aspects and theories of human development. In particular, it will discuss how a certain life event has impacted one’s life, as well as the concept of self-esteem and influential factors of this, and John Bowlbyâ€⠄¢s theory of attachment. Later, several conclusions will be drawn in regards to the participant’s life, such as how they have developed as a human, and what has perhaps enhanced or hindered their development and learning. Research Aim The aim of this essay is to explore the development and learning of a single adult, and attempt to understand this in relation to the impact of certain events on their life. Method Qualitative research will be utilised in order to explore a single adult’sShow MoreRelatedLifespan Development1516 Words   |  7 PagesLifespan Development and Personality Luis Cervantes PSY/103 January 11, 2016 Susanne Nishino Lifespan Development and Personality Developmental psychology is the study of how human beings age and transform throughout the eight major stages of life. This paper will focus on the physical, cognitive, social, moral, and personality development of individuals found in stage two, (early childhood 1-6 year olds). Through exploring, and examining the countless influences that affect their growthRead MoreLifespan Development1327 Words   |  5 PagesLife Roles: Introduction As we learned in Chapter 1 of our text, lifespan development approaches human development from a scientific standpoint. To understand how people go through similar developmental stages and become unique individuals, life developmentalists look at these stages through a framework of cultural, racial, and ethnic differences. This paper will focus on three specific life roles: student, caregiver, and parent, examining the impact of culture, gender, and social status on eachRead MoreLifespan Development945 Words   |  4 Pages* Lifespan development is the field tha examine pattern of growth, change, and stability in behavior. (womb to tomb) * Major topical Areas (Physical Dev., Cognitive Dev., Personality Dev., Social Dev.) * Physical- Body and the brain. * Cognitive- Growth and behavior * Personality- Stability and change * Social- interaction and relationships grow * Cultural factors and developmental diversity * Broad factors * Orientation toward individualism orRead MoreLifespan Development And Communication : Development2151 Words   |  9 Pages Lifespan development and communication – 555101 Individual development narrative Rajbir Kaur 02/05/16 (1877) Introduction One way of looking at human development is through domains of development and how they interrelate. Sigleman and Rider (2003) define development as the systematic changes and continuities that occur between conception and death in an individual. One approach to examining human development is through an individual narrative by integrating theories of developmentRead MoreLifespan Development And Communication Development Essay1536 Words   |  7 Pages Paper: 555101 Lifespan Development and Communications Assessment 3: Individual Development Narrative (IDN) Hoori Wilcox Student ID: 14861456 This assignment identifies and discusses the relevant theory and theorists and discusses the influences possible on lifespan development from the time of conception to 20 years of age. I will analyse my own life story in relation to theories of lifespan development participating my understanding of the domains of development and the age old argumentRead MoreEssay on Lifespan Development1113 Words   |  5 PagesLifespan Development   Ã‚  Ã‚  Ã‚  Ã‚  Going back forty-five years is not an easy task to complete because I can’t remember some of the finer details of my childhood. I know I was born on a hot August afternoon in Birth Year at Place Of Birth in City ands State. My mother was just twenty-two at the time and was already the mother of two, I was her third child. My father was twenty-one and already a workaholic, I know because my mother would constantly remind me not to be like that. My mother and father wereRead MoreThe Development And Development With One s Lifespan857 Words   |  4 PagesGrowth and Development over One’s Lifespan Who We Are is determined by our individual growth and development. According to Erik Erikson (1971) eight stage developmental theory, humans continue to develop throughout their entire lifespan from birth until death. Each stage representing a key aspect in the development of one’s identity, and personality, and overall well-being. Our personal identity gives each of us an integrated and cohesive sense of self that endures and continues to grow as weRead MoreThe Influences On Lifespan Development Essay1780 Words   |  8 PagesHuman development can be defined as growth and change over time that enables an individual to adapt more effectively in their environment (Reference this). The major domains of development include physical, cognitive, emotional and moral. The aim of this paper is to analyse the influences on lifespan development from the time of conception through to 18 years of age. This can be achieved by understanding the domains of development and relating the t heories of lifespan development to real life examplesRead MoreDevelopment over the Lifespan3149 Words   |  13 Pages| Development Over the Lifespan | Third Quarter Psychology Project | | Christina Chantharavongsa | 3/24/2013 | | My full name is Christina Kaysone Chantharavongsa and I was born March 23, 1995 in Bridgeport Hospital located in Bridgeport, Connecticut. It was a little city and a small world since a ton of people would know each other. My parents were in an arranged marriage and were both moved to the United States from Laos during their high school years. But I’m actually half LaotianRead MoreLifespan Development : Cognitive, And Personal And Social Development1717 Words   |  7 PagesLifespan Psychology Introduction Lifespan Development is the field of study that examines patterns, modes of growth, change, and stability in behavior that occur throughout the entire lifespan of an individual. There are so many things to keep in mind about Lifespan Psychology; 1. Lifespan Psychology is a scientific, developmental approach that focuses on human development. 2. Scientists who study the lifespan know that neither heredity nor environment alone can account for the full range of human

The Impact Of Water Pollution - 1487 Words

Water quality is a necessity to human life and all other species. According to Turk, J., Bensel, T. (2014) the Clean Water Act The primary federal law in the United States governing water pollution, whose purpose is to restore and maintain the chemical, physical, and biological integrity of the nation s waters (Turk, J., Bensel, T. 2014) According to Water Quality and Water-Related Disease â€Å"The global public health impact of water contamination is significant for many reasons: (1) safe drinking water is vital for human survival to prevent dehydration; (2) water is essential for basic hygiene and modern sanitation methods, including wastewater treatment; (3) water use is key to every sector of an industrialized, transitional, and†¦show more content†¦This analysis will test if any contamination exists in manufacture bottle water or tap water. The experiments that I conducted included the following materials, Dasani ® bottled water, Fiji ® bottled water, Jiffy Jui ce, Ammonia test strips, Chloride test strips 4 in 1 test strips, Phosphate test strips, Iron test strips, (3) 250 mL Beakers, (3) 100 mL Beakers, (1) 100 mL Graduated Cylinder, Permanent marker, Stopwatch, Parafilm ®, Pipettes, (3) Foil packets of reducing powder, *Tap. [Heading 2]1 My synopsis amongst three containers of waters Tap Water, Dasani ®, and Fiji ®, tap water has unknown chemicals due to pollutions and oil spills. Dasani and Fiji is a company based product should be purified from contamination. Dasani would probably have a small percentage of pollution due to the inexpensiveness cost, and Fiji would display no contamination as to the expensiveness cost of the product. I believe that the cost plays a significant part in companies product for purified bottled water. I hypothesize that once all the waters tested, the tap water will turn out to be the most contaminated out of Dasani ®, and Fiji ®, while the Dasani will have lowest levels of contaminants, in addition, Fiji will have no levels of contamination leaving it to be the cleanest. Tap Water, Dasani ®, and Fiji ® was dispenser individually into a 100-mL bottle. All three bottles were, personallyShow MoreRelatedWater Pollution And Its Impacts On Water Body1682 Words   |  7 Pages1.1 Back Ground Among all natural resource, water is one of the most important to sustain the living planet, earth and every form of life on its face, including human being. It is an integral part of human’s activities including domestic, agriculture, transport, industry, power generation and recreation. Moreover, the normal uses of human body depends entirely on adequate quantity and quality of water. But pure water does not exist by default in its quality due to natural interaction among the earth’sRead MoreThe Impact Of Freshwater On Water Pollution Essay1547 Words   |  7 PagesStorm Water Pollution 1. Introduction Freshwater is a valuable resource that is required for people to survive. Only 1% of the freshwater on the planet is available for human use (National Geographic, 2015). Therefore water is a precious resource however human actions such as farming and pollution are degrading freshwater sources. This report seeks to find out how Victoria University of Wellington (VUW) contributes to Storm Water pollution and other issues related to Storm Water. Firstly it willRead MoreImpact Of Agriculture On Water Pollution2243 Words   |  9 PagesThe journal article â€Å"Impact of Agriculture on Water Pollution in OECD Countries: Recent Trends and Future Prospects† shed light on the effect of agriculture on our natural water systems. The OECD is the Organization for Economic Co-operation and Development. This organization has over thirty-five countries as its members, and aims to research and uses the research on a wide spectrum of topics to ensure governments foster economic growth, financ ial stability, and overall prosperity of the countryRead MoreEnvironmental Impacts Of Water Pollution1973 Words   |  8 Pagesrelation to water pollution. Water pollution is a problem that has many effects on the environment. Additionally, water is a primary need for all humanity. A factor that has been threatening India’s water quality is poor sewerage and pollution by industries. India has the second largest population globally. As a result, water sources have been strained by the population and residents are unable to access clean water for drinking and daily use. This essay will discuss the environmental impacts that haveRead MoreSources Of Water Pollution And Environmental Impacts1911 Words   |  8 PagesSources of Water Pollution and Environmental Impacts For both countries there are various sources where pollutants found in water are derived. There are two types of water pollution, known as point source and nonpoint source. As defined by Chiras and Reganold, in Natural Resource Conservation: Management for a Sustainable Future, point source has an identifiable source, for example a sewage treatment plant or a factory, which contaminates either surface water or groundwater (283). Nonpoint sourceRead MoreGlobal Water Pollution: Causes, Impacts, and Solutions Essay530 Words   |  3 PagesWater pollution has become a huge problem in many countries all over the world. It is known that water is a very significant factor in life, but if this water becomes contaminated, it will be very dangerous for the humanity and wildlife. Pollution is defined as to make fetid or unclear and dirty (American College Dictionary). So, water pollution means the change in the water composition to be dirty or unclear. This essay will examine the causes of water pollution, the effect of that issue in lifeRead MoreThe Sources, Environmental Impact, and Control of Water Pollution838 Words   |  4 Pages Water pollution is one of the most serious environmental challenges. It occurs when water is contaminated by such substances as human and animal wastes, toxic chemicals, metals and oils. Pollution can affect rain, rivers, lakes, oceans and the water beneath the earth, called ground water. Polluted water may look clean or dirty, but it all contains bacteria, viruses, chemicals or other chemicals than can cause illness or even death. Impurities must be removed beforeRead MoreThe Impact Of River Macroinvertebrates On The Chemical Indices Of Water Quality And Pollution2716 Words   |  11 Pagesammonia or BOD in the water relate to the families of macroinvertebrates present. Different methods have been used to help collect and identify macroinvertebrates and monitor pollution levels. This review mainly focuses on the kick sampling technique for collecting macroinvertebrates. It then describes the methods that are used to collect the levels of ammonia, phosphorus, BOD and nitrogen. Foy et al (2001) wished to access the effectiveness of regulatory controls on farm pollution using chemical andRead MoreThe European Union Law Has A Big Impact On How The Uk Law Monitor Their Water Pollution1878 Words   |  8 PagesThe European Union law has had a big impact on how the UK law monitor their water pollution as there are now specific standards that have to be adhered to as the government are now monitored and measured. The EU first regarded water pollution as an important matter in 1973 and have been introducing new directives since. The directives have looked at a number of topics referring to water pollution. In 2000 the European Commission produced The Water Framework Directive 2000/60/EC (WFD) legislationRead MoreAquatic Ecology : The Science Of Ecology1555 Words   |  7 PagesAquatic ecology is the science of ecology which concentrates on the study of aquatic ecosystems. This field can be broken out into two divisions: freshwater and marine ecology. Understanding aquatic ecosystems is critical since water is the building block to the survival of all life on earth. Aquatic ecology includes the study of aquatic environments including oceans, lakes, ponds, wetlands, rivers, and streams. Every organism needs energy to live, grow and reproduce. In aquatic ecology, biologists